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杭州景杰生物科技有限公司
Anti-ubiquitin rabbit pAb

Cat#:

PTM-1105

Ab Type:

P

Applications:

WB

Species:

Eukaryotes

Price: (100 μl)

2880.00

Price: (20 μl)

864.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by affinity chromatography on immunogen peptide.


Source/Purification

This product is produced by immunizing rabbits with a synthetic peptide corresponding to carboxyl terminus of human ubiquitin protein. Antibodies are purified by peptide affinity chromatography.


Specificity

/

Recommended Applications

ELISA, WB; Not tested in other applications

*WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate ?membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for ?two hours at room temperature with gentle shaking.??

Use at an assay dependent concentration. Optimal ?dilutions/concentrations should be determined by the end user.??

?

Anti-ubiquitin rabbit pAb

???

Western blotting analysis on 30 μg of crude proteins from HeLa, C2C12, COS-7, H1299, 293T and Jurkat whole cell lysates using anti-ubiquitin rabbit pAb (1:2000).


Scientific Description

Ubiquitin (Ub) was first identified in 1975 as a 76 amino acid protein that is highly conserved in all eukaryotic cells. Ubiquitin requires three-types of enzyme to conjugate to other proteins: Ub-activating enzyme (E1), Ub-conjugating enzymes (E2), and Ub-ligases (E3). Ubiquitin can be covalently attached to lysine (Lys) residues of target proteins either as a monomer (monoubiquitin), a polymer (polyubiquitin chains) or a linear polymer (linear polyubiquitin chains). All seven Lys residues (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48, and Lys63) can be ubiquitinated. The ubiquitinated peptides have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw cycles. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.

?

**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.??


ELISA, WB; Not tested in other applications

*WB=Western blotting



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